Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 4EOL Chain:A ((3-291)) ---SMENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRL--ETEGVPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLSMDLKDFMDASLTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 4EOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -179595 for 2244 contacts (-80.0/contact) + 2D Compatibility (PS) -29623 + (NN) -9683 + (LL) 220 1D Compatibility (HY) -38800 + (ID) 9250 Total energy: -266731.0 ( -118.86 by residue) QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4EOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EOL-query.scw PDB file : Tito_Scwrl_4EOL.pdb: