Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPENQSSVSEFLLLGLPIRPEQQAVFFALFLGMYLTTVLGNLLIMLLIQLDSHLHTPMYFFLSHLALTDISFSSVTVPKMLMNMQTQHLAVFYKGCISQTYFFIFFADLDSFLITSMAYDRYVAICHPLHYATIMTQSQCVMLVAGSWVIACACALLHTLLLAQLSFCADHIIPHYFCDLGALLKLSCSDTSLNQLAIFTAALTAIMLPFLCILVSY--------------------GHIGVTILQ-----IPSTKGICKALSTCGSHLSVVTIYYRTIIGLYFLPPSSNTNDKNIIASVIYTAVTPMLNPFIYSLRNKDIKGALRKLLSRSGAVAHACNLNTLGG 3RFM Chain:A ((7-304)) ------------------------SVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADILVGVLAIP-FAITISTGFCAACH-GCLFIACFVLVLAQSSIFSLLAIAIDRYIAIAIPLRYNGLVTGTRAAGIIAICWVLSFAIGLTPMLGWNNCGQP--------GCGEGQVACLFEDVVPMNYMVYFN-FFACVLVPLLLMLGVYLRIFAAARRQLKQMESQPLPGERARSTLQKEVHAAKSAAIIAGLFALCWLPLHIINCF------TFFCPDCSHAPLWLMYLAIVLAHTNSVVNPFIYAYRIREFRQTFRKIIRS---------------

General information: TITO was launched using: RESULT: Template: 3RFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -256397 for 2049 contacts (-125.1/contact) + 2D Compatibility (PS) -26640 + (NN) -1186 + (LL) 3392 1D Compatibility (HY) -29200 + (ID) 3250 Total energy: -313281.0 ( -152.89 by residue) QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_3RFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFM-query.scw PDB file : Tito_Scwrl_3RFM.pdb: