Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRSNHTVTEFILLGFTTDPGMQLGLFVVFLGVYSLTVVGNSTLIVLICNDSHLHTPMYFVVGNLSFLDLWYSSVYTPKILVICISEDKSISFAGCLCQFFFSAGLAYSECCLLAAMAYDRYVAISKPLLYAQAMSIKLCALLVAVSYC----------GGFINSSIITKKTFSFNFCCENIIDDFFCDLLPLVKLACGEKGCYKFLMYFLLASNVICPAVLILASYLFIITSVLRISSSQGRLKAFSTCSSHLTSVTLYYGSILYIYALPRSSYSFDMDKIVSTFYTEVLPMLNPMIYSLRNKDVKEALKKLLP 3RFM Chain:A ((12-206)) ----------------------------VELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADILVGVLAIP--FAITISTGFCAACHGCLFIACFVLVLAQSSIFSLLAIAIDRYIAIAIPLRYNGLVTGTRAAGIIAICWVLSFAIGLTPMLGWNNCGQP--------GCGEGQVACLFEDVVPM-----------NYMVYFNFFACVLVPLLLMLGVYLRIFAAARRQLKQMESQPLPGERARSTLQKEVHAAKSAAIIAGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLAHTNSVVNP

General information: TITO was launched using: RESULT: Template: 3RFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -155503 for 1196 contacts (-130.0/contact) + 2D Compatibility (PS) -17471 + (NN) -452 + (LL) 3220 1D Compatibility (HY) -16400 + (ID) 2550 Total energy: -189156.0 ( -158.16 by residue) QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_3RFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFM-query.scw PDB file : Tito_Scwrl_3RFM.pdb: