Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEII-QATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPFAFEK--------EPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL 1VHN Chain:A ((9-294)) ---------------LAPMAGYTDSAFRTLAFEWG-ADFAFSEMVSAKGFLMNSQKTE---ELLPQPHERNVAVQIFGSEPNELSEAARILSEK-YKWIDLNAGCPVRKVVKEGAGGALLKDLRHFRYIVRELRKSVSGKFSVKTRLG-WEKNEVEEIYRILVEEGVDEVFIHTRTVVQSFTGRAEWKALSVLEK-------RIPTFVSGDIFTPEDAKRALEESGCDGLLVARGAIGRPWIFKQIKDFLRSGKYSEPSREEILRTFERHLELLIK-TKGERKAVVEMRKFLAGYTKDLKGARRFREKVMKIEEV----------------------

General information: TITO was launched using: RESULT: Template: 1VHN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -125573 for 2302 contacts (-54.5/contact) + 2D Compatibility (PS) -29459 + (NN) -11754 + (LL) 4284 1D Compatibility (HY) -14800 + (ID) 3950 Total energy: -181252.0 ( -78.74 by residue) QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_1VHN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHN-query.scw PDB file : Tito_Scwrl_1VHN.pdb: