Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDSRFDEWVQKLKEESFQNNTFDRRKFIQGAGKIAGLSLGLTIAQSVGAFEVNAAPNFSSYPFTLGVASGDPLSDSVVLWTRLAPDPLNGGGMPKQAVPVKWEVAKDEHFRKIVRKGTE------MAKPSLAHSVHVEADGLEPNKVYYYRFKTGHELSPVGKTKTLPAPGANVPQMTFAFASCQQYEHGYYTAYKH--MAKEKLDLVFHLGDYIYEYGPNEYVSKTGNVRTHNSAEIITLQDYRNRHAQYRSDANLKAAHAAFPWVVTWDDHEVENNYANKIPEKGQSVEAFVLRRAAAYQAYYE--HMPLRISSLPNGPDMQLYRHFTYGNLASFNVLDTRQYRDDQANNDGNKPPSDESRNPNRTLLGKEQEQWLFNNLGSS---TAHWNVLAQQIFFAKWNFGTSASPIYSMDSWDGYPAQRERVINFIKSKNLNNVVVLTGDVHASWASNLHVDFEKT--------SSKIFGAEFVGTSITSGGNGADKRADTDQILKEN-PHIQFFNDYRGYVRCTVTP-HQWKADYRVMPFVTEPGAAISTRASFVYQKDQTGLRKVSSTTIQGGVKQSDEVEEDRFFSHNKAHEKQMIKKRAKITN 2QFP Chain:A ((28-417)) -----------------------------------------------------------------------DLVGRAMIISWVTMDEPG---------SSAVRYWSEKNGRKR-IA-KGKMSTYRFFNYSSGFIHHTT--IRKLKYNTKYYYEVGLR-NTTRRFSFITPPQTGL-DVPYTFGLIGDLGQSFDSNTTLSHYELSPKKGQTVLFVGDLSYADRYPNH----------------DNVRWDT-WG-----RFTERSVAYQPWIWTAGNHEIEFAPEINE--------------TEPFKPFSYRYHVPYEASQ----STSPFWYSIKRAS-AHIIVLSSYSAY----------------------GRGTPQYTWLKKELRKVKRSETPWLIVLMHSPLYNSYN----------HHFMEGEAMRTKFEAWFVKYKV--DVVFAGHVHAYERSERVSNIAYKITNGLCTPVKDQSAPVYITIGDAGNYGVID-----SNMIQPQPEYSAFREASFGHGMFDIKNRTHAHFSWNRNQD-----GVAVEADSVWFFNRHW---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2185 -137010 -62.70 -373.32 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -62.70 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2QFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QFP-query.scw PDB file : Tito_Scwrl_2QFP.pdb: