Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKGLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVG-------------RSPGGTTKTRLALTDGVKKSLTEFNRQLQDVSYF----LTDSLPP------DHPYAGIKAAPLIGTAPELWVLGLGEN--SARRAAHQGIGYVFGHFINPERGENAFRIYRESFRPSAHFSNPS-ALFTIFVICAKTDEEAEELALSQDL------WLLRVGKGLDSRVPSIEEAKAHPYTASDKKLIEENRKRMVIGSPTTVKQQ---LLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL 4UWM Chain:A ((16-346)) --------------GRSARQTFDWGIKSAVQADSVGIDSMMISEHASQIWENIPNPELLIAAAALQTKNIKFAPMAHLLPHQHPAKLATMIGWLSQILEGRYFLGIGAGAYPQASYMHGIRNAG----TK-NLNDMVRESLFIMEKIWKREPFFHEGKYWDAGYPEELEDEQHKLADF--SPWGGKAPEIAVTGFSYNSPSMRLAGERNFKPV-SIFSGLDALKRHWEVYSEAAIEAGHTPDRSRHAVSHTVFCADTDKEAKRLVMEGPIGYCFERYLIPIWRRFGMMDGYAKDAGIDPVDADLEFLVD---NVFLVGSPDTVTEKINALFEATGGWGTLQV------------------------

General information: TITO was launched using: RESULT: Template: 4UWM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1233 -108939 -88.35 -386.31 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -88.35 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_4UWM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UWM-query.scw PDB file : Tito_Scwrl_4UWM.pdb: