Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKRTVKNIAEMVKGTLANPQYEQTVIHGVATDTRKLEQHQLFIPLKGERFDGHSFVEQAFEAGVAAVLWDRSVPNPPENHAVILVDDTLTALQQLAKAYLQELGT--RVIGVTGSNGKTTTKDMIHAVLGTQYRVHKTGGNFNNHIGLPLTVLAMPENTEIAVLEMGMSAKGEIDLLSRLANPDAAVITNIGESHMQDLGSREGIAEAKLEIINGLKEDGVLIYIGDE----PLLQNAYSCQTKTYGTGTHNDYQLQDVSQSEEGTHFTIKGIENTFFI--PILGKHNVMNAMAAIAAGAYFGIAPEDAAKGLSGLKVTGMRLELIKTDSGLSIINDAYNASPTSMKAAIQLTESLEGYGKKMLVLGDMLELGDLEETFHKECGAVISPDKIDRVFTYGKLGAFIAEGALKHFEKDRVSHYTEK--KDLLQAVKENASKGDLILFKASRGMKLEEIVKDLIESPLS 4ZIY Chain:A ((39-472)) --------------------QIPQTEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVVANGCQVAIVERPID---AEIAQLVVADTRLALGQLG-AYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAH---FGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIREAAKSHQIMSFGAG--GDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQKTPHL-FIDDTYNANPTSMRAAAQVL--LQQNGIKVMVMGDIGEL---SWQEHHDLGRDLAELPLDHIVAVGQFASAALEG-----TKLKAFQTQAEALPFLINLIQTH--QSMSFLFKGSRFTHMETLMADLME----

General information: TITO was launched using: RESULT: Template: 4ZIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2306 -242039 -104.96 -588.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -104.96 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4ZIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZIY-query.scw PDB file : Tito_Scwrl_4ZIY.pdb: