Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKLAVCLSIVLLLLGAASPISAHTVYPV--NPNAQQTTKDIMNWLAHLPNRSENRVMSGAFGGYSDVTFSMTEENRLKNATGQSPAIYGCD---YGRGWLETADITDTIDYCCNSSLISYWKSGGLPQVSLHLANPA---------FPSGNYKTAISNSQYKNILDPSTVEGKRLEALLSKIADGLTQLKNQGVTVLFRPLHEMNGEWFWWGLTGYNQKDNERISLYKELYKKIYRYMTETRGLDNLLWVYSPDANRDFKTDFYPGSSYVDITGLDAYFTDPYAISG-YDEMLSL---KKPFAFAETGPSGNIGSFDYAAFINAIRQKYPQTTYFLTWDEQLSPAANQGAQSLYQNSWTLNKGEIWNGGSLTPIAE 3ZM8 Chain:A ((128-397)) -------------ITLTPSAPRPPHDINPNLNNPNADTNAKKLYSYLRSVYG---NKIISG--------QQELHHAEWIRQQTGKTPALVAVDLMDYSPSRVERGTTSHAVE-----DAIAHHNAGGIVSVLWHWNAPVGLYDTEENKWWSGFYTRATDFDIAATLANPQGANYTLLIRDIDAIAVQLKRLEAAGVPVLWRPLHEAEGGWFWWGAKGPEPA--------KQLWDILYERLTVHHGLDNLIWVWN-----SILEDWYPGDDTVDILSADVYAQGNGPMSTQYNELIALGRDKKMIAAAEVGAA------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1168 -94616 -81.01 -375.46 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -81.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_3ZM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZM8-query.scw PDB file : Tito_Scwrl_3ZM8.pdb: