Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSMDHRIERLEYYIQ--------LLVKTVDMDRY------PFYALLIDKGLSKEEGEAVMRICDELS---EELATQKAQ-------GFVTFDKLLALFAGQLNEKLDVHETIFALYEQGLYQELMEVFIDIMKHFD 3EFO Chain:B ((140-266)) ---LDHIGRRLDHYEKPELSLGSYEYVATLDYCRKSKPPNPPAFIFMIDVSYSNIKNGLVKLICEELKTMLEKIPKEEQEETSAIRVGFITYNKVLHFFNVKSN---------LAQPQMMVVTDVGEVFVPLLDGF-

General information: TITO was launched using: RESULT: Template: 3EFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 210 -44654 -212.64 -446.54 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -212.64 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.080

(partial model without unconserved sides chains): PDB file : Tito_3EFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EFO-query.scw PDB file : Tito_Scwrl_3EFO.pdb: