Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFLIGANGQIGQRLVSLFQDNPDHSI-RAMVRKEEQKASLEAAGAEA-VLANLEGSPEEIAAAAKGCDAIIF--TAGSGGSTGYDKTL---------------LVDLDGAAKAIEAAAIAGIKRFIMVSALQAHNRENWNEALKP--YYVAKHYADKILEASGLTYTIIRPGGLRN-EPGTGTVSAAKDLE-----RGFISRDDVAKTVIASLDEKNTENRAFDLTEGDTPIAEALKKL 2Q46 Chain:A ((7-228)) --VLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGK---EKIGGEADVFIGDITDADSINPAFQGIDALVILTSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIGQKNQIDAAKVAGVKHIVVVGSMGGTNPDHPLNKLGNGNILVWKRKAEQYLADSGTPYTIIRAGGLLDKEGGVRELLVGKDDELLQTDTKTVPRADVAEVCIQALLFEEAKNKAFDL--------------

General information: TITO was launched using: RESULT: Template: 2Q46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 968 -92283 -95.33 -473.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -95.33 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_2Q46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q46-query.scw PDB file : Tito_Scwrl_2Q46.pdb: