Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSMLDTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDV--VLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVI---SPYRTQTPKDVPVTEEELKADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSERLYSFLQNGTFVLLSLRQEADDHIEVKG----LRTVTASL--AEPNEKLRNVHTILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 2QA2 Chain:A ((16-495)) -------IVVGAGPAGLMLAGELRLGGVDVMVLEQLPQRTGESRGLGFTARTMEVFDQRGILPAFGPVETST-QGHFG--GRPVDFGVLEGAHYGVKAVPQSTTESVLEEWALGRGAELLRGHTVRALTDEGDHVVVEVEGPDGP-RSLTTRYVVGCDGGRSTVRKAAGFDFPGTSASREMFLADIRGCEITPRPIGE---TVPLGMVMSAPLGDGVDRIIVCERGAPARRRTG---PPPYQEVAAAWQRLTGQDISHGEPVWVSAFGDPARQVSAYRRGRVLLAGDSAHVHLPAGGQGMNVSVQDSVNLGWKLAAVVSGRAPAGLLDTYHEERHPVGRRLLMNTQAQGMLFLSGDEMQPLRDVLSELIRYDEVSRHLAGMVSGLDIRYEVDGGDHP--LLGMRMPHQELVRAHGKTS-TTELLHPARGVLLDIADDAEVREAATGWSDRVDIVTASLHDAPPQGPLSDARAVLVRPDGYVAWI----SPGSRAGLTEALDRWF------

General information: TITO was launched using: RESULT: Template: 2QA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2694 -242139 -89.88 -516.29 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -89.88 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_2QA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QA2-query.scw PDB file : Tito_Scwrl_2QA2.pdb: