Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKALKALGVLTTFVMLIVLIGGALVTKTGSGQGCGRQWPLCHGRFFPELNPASIIEWSHRFASGISIILVLSLAFWSWRKITPIFRETTFLAIMSIIFLFLQALLGALAVVFGSNALIMALHFGISLISFASVLILTLLIFEADKSVRTLVKPLQIGKKMQFHMIGILIYSYIVVYTGAYVRHTESSLACPNVPLCSPLNNGLPTQFHEWVQMGHRAAALLLFVWIIVAAVHAITSYK-DQ---------------------------KQIFWGWISCLIFITLQALSGIMIVYSEL----A----LGFALAHSFFIACLFGVLCYFLLLIARFRYESRQS 1KQF Chain:C ((15-165)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------RACHWTVVICFFLVALSGISFFFPTLQ-----WLT--Q--T-F--GTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHKVADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGIILIHA------------------

General information: TITO was launched using: RESULT: Template: 1KQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 362 -94492 -261.03 -821.67 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain C : 0.49 3D Compatibility (PKB) : -261.03 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_1KQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KQF-query.scw PDB file : Tito_Scwrl_1KQF.pdb: