Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVISGSKKGRSLKAVAGTSTRPTTDKVKESIFNMIGPYFD--GGRGLDLFAGSGGLGIEALSRGFEHCIFVDRDFKAIQTVKSNLKTLELTKHAQVYRNDAERALHAAAKRETGFRGIFLDPPYK--EQKLKALLTLIDEYQMLEEDGFIVAEHDREVELPETVGDLVMTRKETYGLTGVAIYKKRG 3P9N Chain:A ((4-153)) -RIIGGVAGGRRI-AVPPRG---TTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLEALSRGAASVLFVESDQRSAAVIARNIEALGLS-GATLRRGAVAAVVAAGTTSPVDL--VLADPPYNVDSADVDAILAALGTNGWTREGTVAVVER---------------------------------

General information: TITO was launched using: RESULT: Template: 3P9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 713 -80605 -113.05 -563.67 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -113.05 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_3P9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P9N-query.scw PDB file : Tito_Scwrl_3P9N.pdb: