Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAHTNTQLSQWLESKVGQTLDIRKGELTHD--EEISDLDQIVLHLQKVAIRSTNHPDDYVAKEEL-----VLEGEGTTFTEDGNVPLPQNAYEIPLLGELHIHQENEGLKVVTDRAVYTIDIQHS 3EA5 Chain:B ((535-616)) VAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIED-------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 158 -15478 -97.96 -206.37 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -97.96 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_3EA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EA5-query.scw PDB file : Tito_Scwrl_3EA5.pdb: