Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKIFIDPGHGGSDPGATGN-GLQEKTLTLQIALALRTILTNEYEG-VSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHVN---SGGGTGFESYIYPDVGAPTTT------------------YQSTIHSEVIQAVDFADRGK--------------------KTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAARAKNAGFDAIVILES 4BIN Chain:A ((174-393)) -IVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLI--EKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVS-------DMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEVAESILAGIKAYF--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 851 -56178 -66.01 -340.47 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -66.01 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4BIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BIN-query.scw PDB file : Tito_Scwrl_4BIN.pdb: