Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALIFLSSLTAIGSSILGRWLGMLDDSYAVGDAWFIG--VLAGLISLLILIDS-------------QTMTKNYIVSLSTILGILGVGFIYFPAAFINILLSITLDKQKKEDLHVR 4LDL Chain:A ((173-246)) --------------------------DAYAADEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHI-------------------

General information: TITO was launched using: RESULT: Template: 4LDL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 60 -9896 -164.93 -176.71 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -164.93 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_4LDL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LDL-query.scw PDB file : Tito_Scwrl_4LDL.pdb: