Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFLLDLFTNWTFDKVLDYTLAAVIWSVFKSRSKQNKYPGYFEKIRRYRNLHPLLRSLSMRVLLSITIHPYMFS 2WWB Chain:L ((3-19)) ----------------------KQSLDVSSDRRKARKAY-----------------------------------

General information: TITO was launched using: RESULT: Template: 2WWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 10 1649 164.90 97.00 target 2D structure prediction score : 0.18 Monomeric hydrophicity matching model chain L : 0.45 3D Compatibility (PKB) : 164.90 2D Compatibility (Sec. Struct. Predict.) : 0.18 1D Compatibility (Hydrophobicity) : 0.45 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_2WWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WWB-query.scw PDB file : Tito_Scwrl_2WWB.pdb: