Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRQVKCPYCETKLDKDSAIPYKKRYYHEKCFNT-WKQESDHRKELIQYICNLYGLTSPTGMMLKQIKEF------QEEYGYKLKGIELALRYFYETLDNQPREGDGIGIVPFVYDEAKRHYIRQKAIRKSAEDPKNHKREEITLVIKKGMRKKRGLVDISML 4LY7 Chain:A ((31-137)) ---------------------------HEKTFSAGFHLTTNNRMELMAAIVALESLKQPCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVDLWQRLDAAI----------QRHTINWKWVKGHAGHPENERCDELA-------------------

General information: TITO was launched using: RESULT: Template: 4LY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 308 12689 41.20 126.89 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 41.20 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.036

(partial model without unconserved sides chains): PDB file : Tito_4LY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LY7-query.scw PDB file : Tito_Scwrl_4LY7.pdb: