Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVIKGLTAGLIFLFLCACGGQQIKDPLNYEVEPFTFQNQDGKNVSLESLKGEVWLADFIFTNCETICPPMTAHMTDLQKKLKAENIDVRIISFSVDPENDKPKQLKKFAANYPLSFD-NWDFLTGYSQSEIEEFALKSFKAIVKK---PEGEDQVIHQSSFYLVGPDGKVLKDYNGVENTPYDDIISDVKSASTLK 4WBR Chain:D ((5-154)) ---------------------------------PFQLTDQNGKAVTDKSLKGKPTLIFFGYTHCPDVCPTSLFEISEVLRAMGKDADKVNAIFISVDPERDTPATMK----NYLSSFDPHLEGLSG-DPAEIAK-VITSYRVYAKKVPTKDGDYTMDHTALIYLMDRDGRFVSPFN-LKRTP-EEAAADLK------

General information: TITO was launched using: RESULT: Template: 4WBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 692 -20130 -29.09 -137.87 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.71 3D Compatibility (PKB) : -29.09 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_4WBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WBR-query.scw PDB file : Tito_Scwrl_4WBR.pdb: