Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLRPHLKIEAKGTGSVSFFSEDWLTAQQARTFARELGRFPYMKELEFEDEKGGSWTLKELEKLTEELAQEPDDITVYFDGSFDKESELAGLGIVIYYSLGGTRHRLRKNKSFRLKTNNEAEYAALYEAIREVRELGASRNSITIKGDSLVVLNQLDGSWPCYDPSHNEWLDKIEALLESLKLTPTYETIQRKDNQEADGLAKKILSHQFVESHTKLDRNGDDDIG 4E19 Chain:A ((70-196)) --------------------------------------------------------------------------VHAYFDGASRGNPGPAAVGWVLVSGDGGIV--AEGGDTIGRATNNQAEYDALIAALEAAADFGF--DDIELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFD-DWSITHVPRATNERADALANEAL--------------------

General information: TITO was launched using: RESULT: Template: 4E19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 667 -37106 -55.63 -292.17 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -55.63 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_4E19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E19-query.scw PDB file : Tito_Scwrl_4E19.pdb: