Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRGKGMKFVGDSRIPAEKKPNIPKDYSEYPGKTEAFWPNFLLKEWMVGAVFLIGFLVLT-----IVHQPPLERMADPTDTGYIPLPDWYFLFLYQLLKYEYAAG----------SFTVVGAMIMPGLA-FGALL---LAPFLDRGTERRPWKRPVAVGMMLLAISAAVFLTW----QSVATHDWAKAEEQGKITKEADIDTNAEGYKVFKEQGCISCHGDNLQGGAAGPSLVDSGLKPDEIKKIAVEGKGKMPAGVFKGNDKQLEELAKFISETTAK 2QJP Chain:A ((239-389)) ---------------------------------TVPFWPYFIIKDVFALAVVLLVFFAIVGFMPNYLGHPDNYIEANPLSTPAHIVPEWYFLPFYAILR-AFTADVWVVQIANFISFGIIDAKFFGVLAMFGAILVMALVPWLDTSPVRSGRYRPMFKIYFWLLAADFVILTWVGAQQTTFPYDW---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QJP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 -20918 -105.12 -163.42 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -105.12 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.059

(partial model without unconserved sides chains): PDB file : Tito_2QJP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QJP-query.scw PDB file : Tito_Scwrl_2QJP.pdb: