Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQT-----VTVSANAVRT-----EGSAI-YLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAM-KLKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGIDLIAVTINDPND---WDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDI----TYLLTEEEKENVKINTTLLKPKKAWEKDASKIPDIVGHMEIMFNDATIAKVP-IYYENERHQKPKKQFFETFKSIFLNAAGGAKWSI 3IT9 Chain:A ((8-347)) ---------------------------APSVDARAWILMDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAKKLGLTNTTFQTVHGL-DAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQ--PNRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASYTLTEPQLT---APLKKGQVVGTIDFQLNGKSIEQRPLIVMEN-----------------------------

General information: TITO was launched using: RESULT: Template: 3IT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1799 46476 25.83 145.24 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 25.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3IT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IT9-query.scw PDB file : Tito_Scwrl_3IT9.pdb: