Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLGIDMNVKESVLCIRLTGELDHHTAETLKQKVTQSLEKDDIRHIVLNLEDLSFMDSSGLGVILGRYKQIKQIGGEMVVCAISPAVKRLFDMSGLFKIIRFEQSEQQALLTLGVAS 1TIL Chain:B ((4-119)) MSLAIDLEVKQDVLIVRLSGELDHHTAEELREQVTDVLENRAIRHIVLNLGQLTFMDASGLGVILGRYKQIKNVGGQMVVCAVSPAVKRLFDMSGLFKIIRVEADEQFALQALGVA-

General information: TITO was launched using: RESULT: Template: 1TIL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 571 -109405 -191.60 -943.15 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.94 3D Compatibility (PKB) : -191.60 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.643

(partial model without unconserved sides chains): PDB file : Tito_1TIL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIL-query.scw PDB file : Tito_Scwrl_1TIL.pdb: