Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMFVESINDVLFLVDFFTIILPALTAIGIAFLLRECRAGEQWKSKRTDEHQTVFHINRTDFLIIIYHRITTWIRKVFRMNSPVN-DEEDAGSLLL 2J9W Chain:A ((178-205)) -------------------------------------------------------------------EKVSQWLQKLSSMSASDELDDSQVRQML-

General information: TITO was launched using: RESULT: Template: 2J9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 131 5.02 4.83 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : 5.02 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_2J9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J9W-query.scw PDB file : Tito_Scwrl_2J9W.pdb: