Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKIGYRTIKTALGTALAIYISQLLHLQNFASAGIITILCIQITQKRSLQASWARFSACCLAIAFSYLFFELIGYHPFVIGALLLIFIPITVLLKINEGIVTSSVIILHLYMSGGITPTFIWNEVQLITVGIGVALLMNLYMPSLDRKLIAYRKKIEDNFAVIFAEIERYLLTGEQDWSGKEIPETHQLITEAKNLAYRDVQNHILRYENLHYHYFKMREKQFEIIERLLPKVTSISITVDQGKMIAEFIH-------DLREAIHPGNTAYKFLKRLADMRKEFEEMPLPATREEFEARAALFHLLGEMEQYLVIKSYFKGIKAQKSLG 1RV4 Chain:A ((405-479)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PALTSRGLLEKDFQKVAHFIHRGIELTVQIQDDTGPRATLKEFKEKLAGDEKH--QRAVRALRQEVESFAALFPLPG----------------------

General information: TITO was launched using: RESULT: Template: 1RV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 114 2872 25.19 42.24 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : 25.19 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_1RV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RV4-query.scw PDB file : Tito_Scwrl_1RV4.pdb: