Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKAEALAHTVIYRKNSRFDKVKEKSEELTLIGKGRSAYVFALTE--GGRKMALKVFF----PEYQATAVKEAAIYEKLAGS---AFYPDIYETGDSFILMEYIKGETFYNCLKKGIAISDDMIQQVEEALSDA-----RAAGLNPSDIHLRNLILTETGAVRVIDVARFEQTKTCTQWDDLKSAYHALYKKPIFPKKIPGFWLEIIAFLYKKDWFQKHFAQRKRKYS 3ZM4 Chain:A ((29-207)) ---------------KDDDFEKISE-------LGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCD--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 424 -9373 -22.10 -71.55 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -22.10 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3ZM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZM4-query.scw PDB file : Tito_Scwrl_3ZM4.pdb: