Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAKRQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTFEYC-KNRKYTPKGEEFDKA--VEEWK-ALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDKKEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRA----IVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSM-NNDVVPEGER--CASTSNRNFEGRQGKGARTH--LVSPAMAAMAAIHGHFVDVRKFYQEKTVV 1C97 Chain:A ((143-475)) ---------------------------------------------------------------------------------------------------------GIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSV-------FPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDLAHPV-AEV-------------GSVAEKEGWPL-------DIRVG--LIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAG---------------

General information: TITO was launched using: RESULT: Template: 1C97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1854 -38351 -20.69 -119.85 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -20.69 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_1C97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1C97-query.scw PDB file : Tito_Scwrl_1C97.pdb: