Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAERGASPKQIKPHRYYLLAVH 3JAM Chain:N ((38-50)) IKYARKGLTPSQI-----------

General information: TITO was launched using: RESULT: Template: 3JAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 12 -1017 -84.75 -78.23 target 2D structure prediction score : 1.00 Monomeric hydrophicity matching model chain N : 0.67 3D Compatibility (PKB) : -84.75 2D Compatibility (Sec. Struct. Predict.) : 1.00 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.965

(partial model without unconserved sides chains): PDB file : Tito_3JAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JAM-query.scw PDB file : Tito_Scwrl_3JAM.pdb: