Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNSSTTKPYDE-VLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNILFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHTAGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR 4R5F Chain:A ((2-376)) -AYFDYTSA-KPVDERILEAMLPYMTESFGNPSSVHSYGFKAREAVQEAREKVAKLVNGGGGTVVFTSGATEANNLAIIGYAMRNARKGKHILVSAVEHMSVINPAKFLQKQ-GFEVEYIPVGKYGEVDVSFIDQKLRDDTILVSVQHANNEIGTIQPVEEISEVLAGKAAL--HIDATASVGQIEVDVEKIGADMLTISSNKIYGPKGVGALWIRKEAKLQPVILGGGQENGLRSGSENVPSIVGFGKAAEITAMEWREEAERLRRLRDRIIDNVLKIEESYLNGHPEKRLPNNVNVRFSYIEGESIVLSLDMAGIQASTG--------QPSHVLMACGLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRSM--

General information: TITO was launched using: RESULT: Template: 4R5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2253 20949 9.30 57.24 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 9.30 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4R5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R5F-query.scw PDB file : Tito_Scwrl_4R5F.pdb: