Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVYGPRQRPD--MAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVNIGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE 4M55 Chain:A ((6-307)) -RILITGGAGFVGSHLTDKLMMDG-HEVTVVDNF-----FTGRKRNVEHWIGHENFELINHDVVEP----LYIEVDQIYHLASP-------YNPIKTLKTNTIGTLNMLGLAKRVGA--RLLLASTSEVYG---------------------KHVAETMCYAYMKQEGVEVRVARIFNTFGPRMHMNDGRVVSNFILQALQGEPLTV-----QTRAFQYVSDLVNGLVALMNSN--VSSPVNLGNPEEHTILEFAQLIKNLVGSGSEIQF----------RKPDIKKAKLMLGWEPVVPLEEGLNKAIHYF-------

General information: TITO was launched using: RESULT: Template: 4M55.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1367 -104278 -76.28 -415.45 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -76.28 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4M55.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M55-query.scw PDB file : Tito_Scwrl_4M55.pdb: