Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTVREAKLEDIKDIAKVHVDSWRTT---YHEIIPIDYLNSLNYKEFEDKWKSRSLKGVFVAQDEKGSVFGFASFGPIRSEQEGYDGEL-YAIYLLEERQRQGAGRALLAKGAEFLLQHGFSSMFVWVIEQNPSIIFYQAYSPERVAEDNFEIAGVRLKEVGLG---WPDLSALKTLLNR 2J8N Chain:A ((4-166)) --SIRDAGVADLPGILAIYNDAVGNTTAIWNET-PVDLANRQAWFDA----RARQGYPILVASDAAGEVLGYASYGDWRPF-EGFRGTVEHSVYVRDDQRGKGLGVQLLQALIERARAQGLHVMVAAIESGNAASIGLH----RRLG---FEISG-QMPQVGQKFGRWLDLTFMQLNLD-

General information: TITO was launched using: RESULT: Template: 2J8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 632 -10294 -16.29 -65.98 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -16.29 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2J8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J8N-query.scw PDB file : Tito_Scwrl_2J8N.pdb: