Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELFGVPIQTMYLYTLIIAGSLTLLFLFFGDVFSGLSEGIPFLNPTLVLSFFTCFSAGGYIGELVLPLSSLLIALLSCILSIMLVVLLHIFVLVPLSSAEESLAYREDDLRGRLGKVITAVPVDGFGEVVIEGIGGTISKSAVSFDNQQISYGTTVLVVDINNGVLSVTPHEPI 2EXD Chain:A ((81-142)) -------------------------------------------------------------------------------------------------------GKYTFELKGKVGKVVKIA--EDHYLVEVE----GDKWIAYSD--EKLSLGDRVMVVDVDGLKLKVKRIPP-

General information: TITO was launched using: RESULT: Template: 2EXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 242 -21927 -90.61 -353.65 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.43 3D Compatibility (PKB) : -90.61 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_2EXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EXD-query.scw PDB file : Tito_Scwrl_2EXD.pdb: