Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQAAPE-KKESKPRKPKAAQVSEEAST---PQGFNTLKDKLEEWIEMSNRKDLIKK 4NNH Chain:A ((293-405)) ------IGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELSER---VPDQVVQVGDDAMVKVIDIDLERRRISLSLK--QANEDYTEEFDPSKYGMADSYDEQGNYIFPEGFDP---ETNEWLE----------

General information: TITO was launched using: RESULT: Template: 4NNH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 390 -35737 -91.63 -337.14 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -91.63 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_4NNH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NNH-query.scw PDB file : Tito_Scwrl_4NNH.pdb: