Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRASIVREKKYYELVEQLKDRTQDVTFSATKALSLLMLFSRYLVNYTNVESVNDIN---EECAKHYFNYLMKNHKRLGINLTDIKRSMHLISGLLDVDVNHYLKDFSLSNVTLWMTQER 2XPH Chain:A ((12-81)) ---------DEFPEITEEMEKEIKNVFRNGNQDEVLSEAF-RLTITRKDIQTLNHLNWLNDEIINFYMNMLMERSKEKGL----------------------------------------

General information: TITO was launched using: RESULT: Template: 2XPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 141 -5909 -41.91 -88.19 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -41.91 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.195

(partial model without unconserved sides chains): PDB file : Tito_2XPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XPH-query.scw PDB file : Tito_Scwrl_2XPH.pdb: