Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKHGILNSHLAKILADLGHTDKIVIADAGLPVPDGVLKIDLSLKPGLPAFQDTAAVLAEEMAVEKVIAAAEIKASNQENAKFLENLFSEQE-----------IEYLSHEEFKLLTKDAKAVIRTGEFTPYANCILQAGVLF 3E7N Chain:A ((5-141)) -KKGTVLNSEISSVISRLGHTDTLVVCDAGLPIPNSTARIDMALTQGVPSFMQVVDVVTREMQVEAAILATEIK---QQNPQLHETLLTHLEQLQQHQGNTIKISYTTHEQFKKLTADSQAVIRSGECSPYANVILCAGVT-

General information: TITO was launched using: RESULT: Template: 3E7N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 509 -67177 -131.98 -533.15 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -131.98 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_3E7N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E7N-query.scw PDB file : Tito_Scwrl_3E7N.pdb: