Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLA-VNRSFKNASGEI---EADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTRSYENEEGVNVYVTEVLADTVRFMDPKPREKAAD 3A5U Chain:A ((7-106)) ----ITVVGNLTADPELRFTPSGAAVANFTVASTPRMFDRQSGEWKDGEALFLRCNIWREAAENVAESLTRGSRVIVTGRLKQRSF-TREGEKRTVVEVEVDEI-------------

General information: TITO was launched using: RESULT: Template: 3A5U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 342 -8300 -24.27 -87.37 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -24.27 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3A5U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A5U-query.scw PDB file : Tito_Scwrl_3A5U.pdb: