Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVYVFDHPLIQHKLTYIRNENTGTKDFRELVDEVATLMAFEITRDLPLEEVDI---NTPVQAAKSKVISGKKLGVVPILRAGLGMVDGILKLIPAAKVGHVGLYRDPETLKPVEYYVKLPSDVEEREFIVVDPMLATGGSAVEAIHSLKKRGAKNIRFMCLVAAPEGVEELQKHHSDVDIYIAALDEKLNEKGYIVPGLGDAGDRMFGTK 2EHJ Chain:A ((2-208)) --KIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEATADLETEKVTIEGWNGPVEIDQ---IKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMYRNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLVAAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK

General information: TITO was launched using: RESULT: Template: 2EHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1062 -47963 -45.16 -235.11 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -45.16 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_2EHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EHJ-query.scw PDB file : Tito_Scwrl_2EHJ.pdb: