Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGFKTVVAMVKALMEEYKVTVPVAIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATTAKVVELAHFHGVSVEAELGTVGGQEDDVIAE--GVIYADPKECQELVERTGIDCLAPALGSVHGPYKGE--PNLGFKEMEEIGKSTGLPLVLHGGTGIPT---------------------ADIKKSISLGTAKINVNTENQISSAKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA 2FJK Chain:A ((2-305)) --LVTGLEILRKARAEGYGVGAFNTNNMEFTQAILEAAEEMKSPVILALSEGAMKYGG--RALTRMVVALAQEARV--PVAVHLDHGSSYESVLKALREGFTSVMIDKSHEDFETNVRETKRVVEAAHAVGVTVEAELGRLAGIEEHVAVDEKDALLTNPEEARIFMERTGADYLAVAIGTSHGAYKGKGRPFIDHPRLARIAKLVPAPLVLHGASAVPQELVERFRAAGGEIGEASGIHPEDIKKAISLGIAKINTDTDLRLAFTALVRETLGKNPKEFDPRKYLGPAREAVKEVVKSRMELFGSVGRA

General information: TITO was launched using: RESULT: Template: 2FJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1531 -50777 -33.17 -181.99 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -33.17 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2FJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FJK-query.scw PDB file : Tito_Scwrl_2FJK.pdb: