Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIANYTLKVKGKTL--LQDTDLHFSSGKINHVVGKNGVGKSQLAKDFLLNNSKRI--------GRDIRQ--------NVSLISSSSNIPNDVSKDFLLHFLSKKFDAKMID---KIAYLLN--------LDNIDGK--VLIKNLSDGQKQKLKLLSFLLEDKNIIVLDEITNSLDKKTVIEIHGFLNKYIQENPEKIIINITHDLSDLKAIEGDYYIFNHQEIQQYHSVDKLIEVYINE 3B60 Chain:A ((346-543)) ----NVTFTYPGREVPALRNINLKIPAGKTVALVGRSGSGKSTIAS--LITRFYDIDEGHILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEEYSREQIEEAARMAYAMDFINKMDNGLDTIIGENGVL---LSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDE-LQKNRTSLV--IAHRLSTIE------------------------------

General information: TITO was launched using: RESULT: Template: 3B60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 702 23530 33.52 140.90 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 33.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_3B60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B60-query.scw PDB file : Tito_Scwrl_3B60.pdb: