Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKRITFGLLVLMVCVI-----LFVLYVQLRNKDSIPVQSSAIHPEEDRIFFIYSNPFIKESVLLSTSTGERFNRRTFKVADVPYIQTKSYASTDLVLLAEHEPFYYTLEKDVIKEHPLSDPFAFWYEGKDVTIEAYNVDTTGNEIHINDRKTKKEYTLTLPPLVTMGASDENFIYIIQSMSIYVIDRKTEEMIETLSL---------------------------ASYADQFADSEEFIVASSDHKLTVIEKGTWKTTYIAYPEDLEYADTVYYDKESGSFYVAYEDKEGGANLLEYGEDFSIHTYSLKFPYMEAKF--KGNLLYIVAQEEHKKGIGGYVGVFDIHSKEMLYQFDLP-EEQVKVQDFVVVDNK- 1JJU Chain:B ((1-337)) --RDYILAPARPDKLVVIDTEKMAVDKVITIADAGPTP-MVPMVAPGGRIAYATVNKS--ESLVKIDLVTGETLGRIDLSTP-----EERVKSLFGAALSPDGKTLAIYESPVRLELTHFEVQ-----------------PTRVALYDAETLSRRKAFEAPRQITMLAWARDGSKLYGL-GRDLHVMDPEAGTLVEDKPIQSWEAETYAQPDVLAVWNQHESSGVMATPFYTARKDIDPADPTAYRTGLLTMDLETGEMAMREVRIMDVFYFSTAVNPAKTRAFGAYN----VLESFDLEKNASIKRVPLPHSYYSVNVSTDGSTVWLG-------GALGDLAAYDAETLEKKGQVDLPGNASMSLASVRLFTRDE

General information: TITO was launched using: RESULT: Template: 1JJU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1703 -159137 -93.45 -528.69 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -93.45 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_1JJU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JJU-query.scw PDB file : Tito_Scwrl_1JJU.pdb: