Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MYSTIFNIGQINKYSKLAIFMSILFLCGCSSQTHSSQKETTIPVTLHVEDAKGLPVEGVQVTIVKAPSSDEEPSTEIGEILGKTDKNGDIKWDTGRKGDYSVALTKGETSVTHHISLTEDKKDHAIPLVFKE- 1QUQ Chain:B ((3-116)) DMMDLPRSRINAGMLAQFID-----KPVCFVGRLEKIHPTGK------MFILSDG-----EGKNGTIELMEPLDEEIS-GIVEVVGRVTAKATILCTSYVQ------FKEDSHPFDLGLYNEAVKIIHDFPQFYPLG

General information: TITO was launched using: RESULT: Template: 1QUQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 470 -32172 -68.45 -295.16 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -68.45 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_1QUQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QUQ-query.scw PDB file : Tito_Scwrl_1QUQ.pdb: