Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAGYIMAAENQTLNQWTINQLGITRGDSILEVGFGPGYCMQQMLKRE-KDVHLHGIDVSEAMLKLAARRVKP---KGVRLIQGSIETFPLPASFYDKVISVNNYTIWNDQTKGIKQIYRALKPGGKAAITMQPREADAS-P-EKTKSFGRQMIADFKAAGFEDIDIQFKNIKPELSVCATAKKPAT 3DH0 Chain:A ((29-180)) -----------------VLKEFGLKEGMTVLDVGTGAGFYLPYLSKMVGEKGKVYAIDVQEEMVNYAWEKVNKLGLKNVEVLKSEENKIPLPDNTVDFIFMAFTFHELSEPLKFLEELKRVAKPFAYLAIIDWKKEERDKGPPPEEVYSEWEVGLILEDAGIRVGRVVE------------------

General information: TITO was launched using: RESULT: Template: 3DH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 703 -86755 -123.41 -594.21 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -123.41 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_3DH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DH0-query.scw PDB file : Tito_Scwrl_3DH0.pdb: