Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNERLMLKGIFLG----------AAAGAALSLLHKPTRQACGMRWLT---CKHKLSLYKSNPELLKNTVITKVDEAKKLARTLSKEVDF-VNQQVKELKKTTPQVMELVQETKEHFSKK 1B43 Chain:A ((220-339)) LTREKLIELAILVGTDYNPGGIKGIGLKKALEIVRHSKDPLAKFQKQSDVDLYAIKEFFLNPPVTDNYNLVWRDPDEEGILKFLCDEHDFSEERVKNGLERLKKAIKSGKQSTLESWFKR

General information: TITO was launched using: RESULT: Template: 1B43.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 200 -26773 -133.87 -252.58 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -133.87 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_1B43.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B43-query.scw PDB file : Tito_Scwrl_1B43.pdb: