Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWVSTATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYFWIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTINDQYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVSDAPIVLTNGDELSVTISLGAAHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE 4ZVF Chain:A ((1-163)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MDVLTKLLNRRFLPTIFKREIAHANR-TGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNG-HPDYERLIQIADEALYIAKRRGRNRVELWKAS-

General information: TITO was launched using: RESULT: Template: 4ZVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 748 -105376 -140.88 -646.48 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -140.88 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4ZVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZVF-query.scw PDB file : Tito_Scwrl_4ZVF.pdb: