Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQKPVQWASKIGFVMAAAGSAIGLGAIWKFPYVAGTNGGGAFFLIFVLFTILLGYPLLVGEFIFGR------RNQTNAIDAYKKEAPRSAWFLTGWIGVAACFLVLSFYSVIGGWILLYIVK-----------TASGSLSGLSQAQYGALFASIIQNPV--QTLAAQLVF---MALTVLVVARGVQKGIERVSAVMMPILFLLFILLVLRSL---TLNG-AMEGVKFLLVPHFGDL-TPESILFALGQAFFTLTLGVSVMVTYSSYLPKTQNIPRSAASIVLMNIIVTLLAGLAI-FPAVFSFGFQPNE-------GPTLLFTVLPAVFEQLPFGTLFFIGFLVAFLFAALTSAFSMVEIIVATIGKGDEKKRKK-LSWTSGLLIFLVGIPCCLSYGVLSDVHLFGKTFFDIADFTVSNVLMPSGALLISLFIPLKISKSELLAEMRNGSNAGKAFFYTWFYLLRFIVPLAIIIVFLNLIGILSF 4MM8 Chain:A ((5-394)) -----REHWATRLGLILAMAGYAVDLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAI-FYLLWRNRFAKIL-GVFGLWIPLVVASYYVYIESWTLGFAIKFLVGLVPEPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFSLGLGFGVLITFASYVRKDQDIVLSGLTAATLNEKASVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAGMQGMIAFLEDELKLSRKHAVLWTAAIVFF---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4MM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1721 -285772 -166.05 -828.32 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -166.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_4MM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MM8-query.scw PDB file : Tito_Scwrl_4MM8.pdb: