Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLAGLSREVEENLNKGSWIRKLFDEGARLKKEFGEDQVFDFSLGNPIVEPPEAFKRALIEEAE--KGSHGYIQNQGLLAAREKVAQFLGSRFEADFSA-ERIVMTVGAGGALNVALKSIVNPGEEVIILAPYFAEYKLYIENYGGKAVSCPLTS------RFEIDIEAVRQSITPQTKGLILNTPHNPTGTVLSQKNIDDLGALLKEIEEKSGQTIYVLFDEPYSQLIYD-EELANP---FQSYHRVILASSFSKDLGIAGERLGYIGLDSRMPDADLLINAFVYCNRTLGFVNAPVMMQRAVARMDDLRV-----------D--ASAYKERRDLMVDILKEAGFEFEMPKGGFFVFPKSPIE--------------DEVAFCVHAAQKYKLLIVPSSGFGM-------SGHFRLSFSVPIEQIKNSRDIFISLYKDFA 4WLH Chain:A ((26-421)) ----------------------------------SEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFMLNQYTKTFGYPPLTKILASFFGELLGQEIDPLRNVLVTVGGYGALFTAFQALVDEGDEVIIIEPFFDCYEPMTMMAGGRPVFVSLKPELGSSSNWQLDPMELAGKFTSRTKALVLNTPNNPLGKVFSREELELVASLCQ------QHDVVCITDEVYQWMVYDGHQHISIASLPGMWERTLTIGSAG-TFSATGWKVGWVLGPD----H--IMKHLRTVHQ-NSVFHCPTQSQAAVAESFE-REQLLFRQPSSYFVQFPQAMQRCRDHMIRSLQSVGLKPIIPQGSYFLITDISDFKRKMPDLPGAVDEPYDRRFVKWMIKNKGLVAIPVSIFYSVPHQKHFDHYIRFCFVKDEATLQAMDEKLRKWKVE--

General information: TITO was launched using: RESULT: Template: 4WLH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1961 -243953 -124.40 -713.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -124.40 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4WLH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WLH-query.scw PDB file : Tito_Scwrl_4WLH.pdb: