Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------MNCWKTINLMKDYGAVRIILTAVCFMILVFISTFLAFELLRPGTSLSDEYVSLFGGLLVV-------ILFVHKVIHVLPII-------------------CKKRKIEKKFYILRMRT-WKRIPKTTMLISL----VSPFLLITPVLFYAALA--FPNHAHYFCMISGIHAGYCLPDF--LLALKLIKAPKTAFID-QEADGLDILVEK 1MN4 Chain:A ((63-325)) FKVGPPFELVRDYCPVVESHTGRTLDLRIIPRIDRGFDHIDEEWVGYKRSYFTLVSTFETANCDLDTFLKSSFDLLVEDSSVEGRLRVQYFAIKIKAKNDDDDTEINLVQHTPSVCPLVPSPLPKHQIIREASNVRNITKMKKYDSTFYLHRDHVNYEEYGVDSLLFSYPEDSIQKVARYERVQFASSISVKKPSQQNKHFSLHVILGAVVDPDGIPYDELALKNGSKGMFVYLQEMKTPPLIIRG

General information: TITO was launched using: RESULT: Template: 1MN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 669 -134664 -201.29 -782.93 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -201.29 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_1MN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MN4-query.scw PDB file : Tito_Scwrl_1MN4.pdb: