Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------MADSFLFYNLSEAQMTFQDVMERL------KAFVQKDPRSSYVL-SIGTDSQVYRDYTKFIT------------ALHLHRTGKGAWGCLK-NHTVDRPIHSLREKISLETAYSQETAAHILDGHLMDITDLLLPFTGEGADLTFEVHLDI---GKKGLTKDLIQEMTGRITSMGIEAK---IKPDSYTAFSYANRFTK------------------------- 3U4T Chain:A ((230-487)) VEFRYADFLFKNNNYAEAIEVFNKLEAKKYNSPYIYNRRAVCYYELAKYDLAQKDIETYFSKVNATKAKSADFEYYGKILMKKGQDSLAIQQYQAAVDRDTTRLDMYGQIGSYFYNKGNFPLAIQYMEKQIRPTTTDPKVFYELGQAYYYNKEYVKADSSFVKVLELK-PNIYIGYLWRARANAAQDPDTKQGLAKPYYEKLIEVCAPGGAKYKDELIEANEYIAYYYTINRDKVKADAAWKNILALDPTNKKAIDGLK

General information: TITO was launched using: RESULT: Template: 3U4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 642 -25238 -39.31 -147.59 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -39.31 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.155

(partial model without unconserved sides chains): PDB file : Tito_3U4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U4T-query.scw PDB file : Tito_Scwrl_3U4T.pdb: