Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPEGKIIKALSGFYYVLDESEDSDKVIQCRGRGIFRKNKITPLVGDYVVYQAENDKEGYLMEIKERTNELIRPP----ICNVDQAVLVFSAVQPSFSTALLDRFLVLVEANDIQPIICITKMDLIEDQDTEDTIQAYAEDYRNIGYDVYLTSSKDQDSLADIIPHFQDKTTVFAGQSGVGKSSLLNAISPEL-GLRTNEISEHLGRGKHTTRHVELIHTS-GGLVADTPGFSSLEFTDIEEEELGYTFPDIREKSSSCKFRGCLHLKEPKCAVKQAVEDGELKQYRYDHYVEFMTEIKDRKPRY 2RCN Chain:A ((48-345)) -AEGIVISRFGMHADVESAD---GEVHRCNIRRTIR----SLVTGDRVVWRPGKV-KGIVEAVHERTSVLTRP-VKPIAANIDQIVIVSA-ILPELSLNIIDRYLVGCETLQVEPLIVLNKIDLLDDEGM-DFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGVGKSSLLNALLGLQNEILTN-------------TAARLYHFPHGGDVIDSPGVREFGLWHLEPEQITQGFVEFHDYLGHCKYRDCKHDADPGCAIREAVENGAIAETRFENYHRILESMA------

General information: TITO was launched using: RESULT: Template: 2RCN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1360 -197903 -145.52 -741.21 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -145.52 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_2RCN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RCN-query.scw PDB file : Tito_Scwrl_2RCN.pdb: