Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNILKNWKNQQTAASNLERYTKEDILKGEIPEHIAIIMDGNGRWAKKRSLPRIAGHHEGMKVVKRTTKLANELGVKVLTLYAFSTENWKRPKMEVDFLMKLPEEFLNTYLPELV--EENVQVRIIGDETALPAHTLRAIEKAVQDTAQNDGMILNFALNYGGRTEIVSAAKSLAEKVKEGSLNIEDIDESLFSTYLMTE--SLQDPELLIRTSGEIRLSNFMLWQVAYSEFVFTDVLWPDFKEDHFLQALGEFQQRGRRFGGI 5HC8 Chain:A ((13-243)) -------------------------------PNHVAIIIDGHRKWAKSRGVTVQEGHQTGVNNWKHIISRASQLGIKLLTIWALSPQNFNRSKMEVDFLMRIYEDFLRSDVKELVTSQQDIQFSAIGDKSRLPEYLQDAISYAEGLSQANKGMHFILAVAYGGREDIVEAARKIAAKVEHGILRPDDIDEATFEQHLMTNITKFPSPDLLIRAAGEQRLSNFFLWQLPFTEFYFTPKLFPDFGEADLLDALASYRCRYRGFG--

General information: TITO was launched using: RESULT: Template: 5HC8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1117 -123502 -110.57 -544.06 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -110.57 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_5HC8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HC8-query.scw PDB file : Tito_Scwrl_5HC8.pdb: